Genetic Variation and Composition of Two Commercial Estonian Dairy Cattle Breeds Assessed by SNP Data.

The aims of this study were to assess the genomic relatedness of Estonian and selected European dairy cattle breeds and to examine the within-breed diversity of two Estonian dairy breeds using genome-wide SNP data. This study was based on a genotyped heifer population of the Estonian Red (ER) and Estonian Holstein (EH) breeds, including about 10% of all female cattle born in 2017-2020 (sample sizes n = 215 and n = 2265, respectively). The within-breed variation study focused on the level of inbreeding using the ROH-based inbreeding coefficient. The genomic relatedness analyses were carried out among two Estonian and nine European breeds from the WIDDE database. Admixture analysis revealed the heterogeneity of ER cattle with a mixed pattern showing several ancestral populations containing a relatively low proportion (1.5-37.0%) of each of the reference populations used. There was a higher FROH in EH (FROH = 0.115) than in ER (FROH = 0.044). Compared to ER, the long ROHs of EH indicated more closely related parents. The paternal origin of the genetic material used in breeding had a low effect on the inbreeding level. However, among EH, the highest genomic inbreeding was estimated in daughters of USA-born sires.

MAOA methylation is associated with impulsive and antisocial behaviour: dependence on allelic variation, family environment and diet.

Congenital absence of monoamine oxidase A (MAO-A) activity predisposes to antisocial impulsive behaviour, and the MAOA uVNTR low-expressing genotype (MAOA-L) together with childhood maltreatment is associated with similar phenotypes in males. A possible explanation of how family environment may lead to such behaviour involves DNA methylation. We have assessed MAOA methylation and impulsive/antisocial behaviour in 121 males from the Estonian Children Personality Behaviour and Health Study. Of the 12 CpG sites measured, methylation levels at the locus designated CpG3 were significantly lower in subjects with antisocial behaviour involving police contact. CpG3 methylation was lower in subjects with alcohol use disorder by age 25, but only in MAOA-H genotype. No correlation between MAOA CpG3 methylation levels and adaptive impulsivity was found at age 15, but in MAOA-L genotype a positive correlation appeared by age 18. By age 25, this positive correlation was no longer observed in subjects with better family relationships but had increased further with experience of adversity within the family. MAOA CpG3 methylation had different developmental dynamics in relation to maladaptive impulsivity. At age 18, a positive correlation was observed in MAOA-L genotype with inferior family relationships and a negative correlation was found in MAOA-H with superior home environment; both of these associations had disappeared by age 25. CpG3 methylation was associated with dietary intake of several micronutrients, most notable was a negative correlation with the intake of zinc, but also with calcium, potassium and vitamin E; a positive correlation was found with intake of phosphorus. In conclusion, MAOA CpG3 methylation is related to both maladaptive and adaptive impulsivity in adolescence in MAOA-L males from adverse home environment. By young adulthood, this relationship with maladaptive impulsivity had disappeared but with adaptive impulsivity strengthened. Thus, MAOA CpG3 methylation may serve as a marker for adaptive developmental neuroplasticity in MAOA-L genotype. The mechanisms involved may include dietary factors.

Possibility to Estimate Same Day Energy Status of Dairy Cows during First Half of Lactation by Non-Invasive Markers with Emphasis to Milk Fatty Acids.

Postpartum negative energy balance (NEB) is detrimental to cows and decreases profitability in dairy farming. The two origins of milk fatty acids (FA), de novo synthesized in the mammary gland and plasma lipids initially originating from feed, rumen microbes and the animal's adipose tissue, make milk FA candidates as possible NEB biomarkers. The aim of this study was to assess the possibility to predict EB in cows in the first 150 days of lactation with BCS, milk traits and selected individual milk FA and the ratios of blood-derived and de novo synthesized FA. The daily EB of Estonian Holstein cows (N = 30) was calculated based on body weights and BCS values. Milk FA were analyzed with gas chromatography. The variance partitioning analysis revealed that milk production traits, BCS at calving, FA ratios and days in milk accounted for 67.1% of the EB variance. Random forest analysis indicated the highest impact of the ratios C18:1cis9/C12:0+C14:0, C18:1cis9+C18:0/C12:0+C14:0, C18:1cis9/C14:0, C18:1cis9+C18:0/C14:0, C18:1cis9/sum C5:0 to C14:0, C18:1cis9+C18:0/sum C5:0 to C14:0 or C18:1cis9/C15:0. FA and their ratios alone explained 63.6% of the EB variance, indicating the possibility to use milk FA and their ratios as sole predictors for the energy status in dairy cows.

Changes in dairy cows' behaviour, health, and production after transition from tied to loose housing.

BACKGROUND: Transition of dairy cows from a tied to a loose housing system may affect their behaviour, health and production. Such housing system changes have become more frequent in Estonia but knowledge is lacking on how cows adapt to a new system. The aim of this study was to evaluate how cows' behaviour, milk production and composition, and different aspects of their health changed after transition from tied to loose housing. RESULTS: A herd of 400 dairy cows was moved to a new system on the same farm, so that effects of transport were not confounding factors. Behavioural observations were made for approximately 4 months following transition. Milk production data were recorded from 12 months before to 12 months after transition. Examination for skin alterations and cleanliness, as well as body condition scoring were carried out before transition, and thereafter monthly throughout the study. Significant effects on behaviour were observed just after the transition, with increases in the behaviour indicative of poor welfare, such as vocalisation and aggression, and decreases in those indicative of a good state of welfare, such as ruminating, resting and grooming. These effects were of short duration, with most returning to a steady state after the first week. Milk production declined already before the transition but fell significantly after transition, and this fall lasted longer in older cows. Likewise, somatic cell counts were higher in all cows following transition, but older cows were affected significantly more than cows in the first lactation. The frequency of lameness and skin alterations increased on average after transition. Body condition scores fell after transition but recovered by the second month. Therefore, there were adverse effects on the behaviour, health and production of the dairy cows transferred, although, apart from older cows, of short duration. CONCLUSION: The transition from tied to loose housing first had negative impacts on the welfare of the cows, although by the tenth day the behavioural indicators had returned to normal values. Impacts were more severe in higher parity cows, indicating that the change was more of a challenge for older cows. The findings of this study suggest that animals' behaviour and health should be more carefully observed within about 2 weeks after transition. It is quite likely that more and more farmers in Estonia and elsewhere will recognize the benefits of keeping their dairy cattle in loose housing, aimed at improving animal welfare and the value of the production chain.

Spatial and intra-host distribution of myxozoan parasite Tetracapsuloides bryosalmonae among Baltic sea trout (Salmo trutta).

Proliferative kidney disease caused by the myxozoan parasite Tetracapsuloides bryosalmonae has been actively studied in juvenile salmonids for decades. However, very little is known about parasite prevalence and its geographical and intra-host distribution at older life stages. We screened T. bryosalmonae among adult sea trout (Salmo trutta) (n = 295) collected along the Estonian Baltic Sea coastline together with juvenile trout from 33 coastal rivers (n = 1752) to assess spatial infection patterns of the adult and juvenile fish. The parasite was detected among 38.6% of adult sea trout with the prevalence increasing from west to east, and south to north, along the coastline. A similar pattern was observed in juvenile trout. Infected sea trout were also older than uninfected fish and the parasite was detected in sea trout up to the age of 6 years. Analysis of intra-host distribution of the parasite and strontium to calcium ratios from the otoliths revealed that (re)infection through freshwater migration may occur among adult sea trout. The results of this study indicate that T. bryosalmonae can persist in a brackish water environment for several years and that returning sea trout spawners most likely contribute to the parasite life cycle by transmitting infective spores.

Active vs passive novelty-related strategies: Sex differences in exploratory behaviour and monoaminergic systems.

Sex differences are apparent in numerous behavioural characteristics. In order to compare and characterise male and female variability of exploratory behaviour, 365 male and 401 female rats were assessed in a task where a bimodal response distribution had previously been established in males. Female rats had significantly higher exploratory activity, and presented normal distribution of the behaviour, very differently from the bimodal distribution of males. No major effect of litter or oestrous cycle was detected. Several differences between male and female rats were found in monoamine metabolism measured ex vivo. Male rats had lower levels of dopamine (DA) in frontal cortex, and higher levels of 3,4-dihydroxyphenylacetic acid (DOPAC) in raphe area; higher levels of serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) in dorsal striatum but lower levels of 5-HT and 5-HIAA in locus coeruleus area, 5-HIAA levels were also lower in hippocampus as compared to females. Males had higher noradrenaline (NA) levels in hippocampus and lower normetanephrine (NMN) levels in striatum, in both brain regions male animals had lower NMN/NA ratio. No sex difference was found in accumbens. The only brain region with an interaction between sex and the expression of exploratory activity was raphe: Here 5-HT levels were lower, and DOPAC levels and DOPAC/DA and 5-HIAA/5-HT ratios higher in low exploring male but not female rats. Conclusively, female rats not only display higher levels of exploration but the population distribution of this behaviour is distinct; this may be related to differences in the monoaminergic systems between female and male animals.

Calf care workers' attitudes and personality and their association with calf mortality in large-scale dairy farms.

Although calf mortality is a multifactorial problem, little is known about the attitudes and personalities of calf care workers (CCWs) and their association with calf mortality. This study aimed to describe the attitudes, satisfaction, and personality of CCWs in large Estonian dairy herds and to analyze their associations with herd calf mortality. A questionnaire registering CCWs' attitudes toward their work and calf mortality, personality characteristics, satisfaction and importance of different job-related factors was developed. In total, completed questionnaire data of 161 CCWs from 108 large (>100 cows) Estonian dairy farms were analyzed. Herd-level yearly calf mortality risk (MR) was calculated. Cluster analysis and variance partitioning analysis were applied to reveal the explanatory capacity of CCWs' attitudes and personalities on calf mortality. The mean yearly herd-level calf MR was 5.4% during the first 21 days of life and 2.7% during 22-90 days of life. Although good calf health and low calf mortality was important for CCWs, dead calves were often seen as inevitable. CCWs were generally doubtful regarding their capacity and available knowledge to influence calf mortality. In high-mortality herds, CCWs were dissatisfied with the calf health situation and farm working equipment and felt that the situation was out of their control. Despite striving, they had less faith that farmworkers could affect the outcomes, such as calf mortality. CCWs' personality domains explained <5% of the variance in the herd's calf MR, whereas their attitudes and satisfaction explained 20% of the variability in calf MR. The current study revealed the importance of the attitudes and satisfaction of CCWs on calf mortality and highlighted the need to allocate proficient assistance to herds with high calf mortality to mitigate calf health problems and the resulting consequences for CCWs.

Faecal microbiota in two-week-old female dairy calves during acute cryptosporidiosis outbreak - Association with systemic inflammatory response.

In the present study, relationships between the intestinal microbiota and innate immunity response, acute cryptosporidiosis, and weight gain in female dairy calves were investigated. A total of 112 calves born during a natural outbreak of cryptosporidiosis on one dairy farm was included in the study. Microbiota composition was analysed by means of 16S ribosomal RNA gene amplicon sequencing from faecal samples collected during the second week of life, while the status of Cryptosporidium spp. infection was determined using immunofluorescence. Serum samples from the second week of life were colourimetrically analysed for the following markers of acute inflammation: acute-phase proteins (serum amyloid A and haptoglobin) and pro-inflammatory cytokines (interleukin-1 beta, interleukin-6, and tumour necrosis factor-alpha). Statistical analyses were performed using random forest analysis, variance-partitioning, and negative binomial regression. The faecal microbiota of the two-week old calves was composed of the phyla Firmicutes, Bacteroidetes, Proteobacteria, Fusobacteria, and Actinobacteria (in order of decreasing abundance). Microbial diversity, measured in terms of the Shannon index, increased with the age of the calves and decreased if a high count of Cryptosporidium spp. oocysts was found in the faeces. Fusobacterium was positively associated with Cryptosporidium spp. oocyst count and serum amyloid A concentration. Peptostreptococcus was positively associated with haptoglobin and serum amyloid A concentrations, and negatively associated with average daily weight gain at 9 months of age. The markers of innate immunity, in combination with age, explained 6% of the microbial variation. These results suggest that some components of the intestinal microbiota may have a long-lasting negative effect on animal growth through the stimulation of the systemic innate immune response.

Bovine colostrum: Postpartum changes in fat globule size distribution and fatty acid profile.

Although "zero waste" valorization concepts are gaining increasing attention, colostrum, a byproduct of milk production, remains underused due to technological challenges. Information about the fat fraction and the size of fat globules is needed to address these challenges, but such information is currently lacking. This study aimed to fill this gap in the knowledge by measuring the size distribution of bovine colostrum fat globules (CFG) and analyzing its relationships with postpartum milkings, parity, and fatty acids (FA) profile. Four sequential postpartum colostrum samples were collected from 44 cows and analyzed for the abovementioned parameters. The results indicated that CFG size increases almost twice during postpartum milkings (from ∼5 to ∼10 µm), whereas lactation has little, if any, effect on CFG size. The FA profile analyses showed that the content of most FA in the fourth postpartum milking was different from the previous milkings. The correlation analyses between CFG size and FA profile also demonstrated that the fourth milking was clearly distinguishable from the first 3 postpartum milkings. For example, the saturated FA content from the first 3 milkings had a positive correlation with smaller CFG (and a negative correlation with larger CFG), whereas the fourth milking demonstrated no correlations. Based on these CFG size and FA profile analyses, the results of this study suggest that the first 3 postpartum milkings can be considered as colostrum, whereas the fourth milking represents transition milk. Information about CFG size distribution enables modification of the FA profile of colostrum products and the ability to create better valorization technologies for colostrum-based food and feed supplements.

Attitudes and personality of farm managers and association with cow culling rates and longevity in large-scale commercial dairy farms.

The farmer has the central role in determining cow culling policies on their farm and thus affecting cow longevity. The present study aimed to examine farm managers´ satisfaction, attitudes, personality traits and analyse the associations with dairy cow culling and longevity in large commercial dairy farms. Farm managers of 116 dairy herds rearing at least 100 cows in freestall barns were included. A questionnaire for the farm managers registered personal background information of respondent and included statements capturing their satisfaction, opinions and attitudes regarding dairy cow culling and longevity, farming in general, and a Ten Item Personality Inventory scoring. For each herd, the last 12 months cow culling rate (CR, excluding dairy sale) and herd mean age of culled cows (MAofCC) was obtained from the Estonian Livestock Performance Recording Ltd. A K-mean clustering algorithm was applied to subgroup farm managers based on their attitudes, opinions and personality traits. The yearly mean herd CR was 33.0% and MAofCC was 60.6 months. Farm managers´ were mostly dissatisfied with cow longevity and culling rates in their farms. Dissatisfaction with culling rates and longevity, priority for producing high milk yields over longevity and production-oriented attitude was associated with high culling rates and poor longevity. Farm managers' personality had an effect on herd culling rates and their attitudes explained one third of the variability of culling rates and longevity. Explaining the economic consequences of high culling rates and decreased longevity, improving the visibility of these parameters together with benchmarking could bring these issues into focus.

The Effects of Vehicle Type, Transport Duration and Pre-Transport Feeding on the Welfare of Sheep Transported in Low Temperatures.

Low temperatures can provide a risk to the welfare of sheep during transport because of increased ventilation chilling the sheep, and we examined the importance of three factors-covering the vehicle, duration of transport, and feeding prior to transport-on the welfare of sixty transported 4-month-old Dorper × Mongolian female sheep in a cold climate. Sheep in a covered vehicle had greater increases in head and ear temperatures than those in an open vehicle. Sheep transported for 2 h increased their leg temperatures, whereas those transported for 1 h had reduced leg temperatures. Increases in non-esterified fatty acids (NEFA) and lactate dehydrogenase (LDH) in the blood samples during the long transport suggested that sheep had more muscular and metabolic activity, compromising their well-being. Feeding prior to transport did not affect body temperatures, but those not fed prior to transport had reduced alanine transferase, HSP and cortisol in their blood, whereas those that were fed had reduced NEFAs, LDH and creatine kinase. Prior feeding had no effect on the sheep temperature indices over a two-hour transport period. Thus, the sheep most at risk of the adverse effects of cold temperatures were those transported in open vehicles, those transported for a longer time, and those not fed before transport.

Effect of grouping on behaviour of dairy heifers and cows in the transition period.

Regrouping dairy cows is a common feature of dairy farm management. Cows are grouped based on lactation stage, age, milk yield and other factors. Regrouping cows during the dry period (from far-off area to close up area and from close up area to the main herd) brings new challenges. This is especially true for heifers who, after being confirmed gravid, may be grouped into a new pen with dried off cows. The aims of this study were to determine how grouping affects activity, nearest neighbour relationships and aggression, and how heifers' acclimatization to a new group differs from cows. Therefore, the hypotheses were that regrouping cows has less of an effect on older cows compared to heifers, and cows' individuality affects acclimatization to a new group. Aggression data were recorded using a video camera that was directed at the feed bunk, and activity was recorded with activity monitors that were attached around the right hind leg. Synchrony and distance to nearest neighbour were recorded, as was the cows' location on the first 3 d from the day they returned to the main herd. Motion index, mean number of steps and number of lying bouts were significantly higher after calving compared to the week before calving and the difference was higher amongst heifers compared to cows (P < 0.001). Both cows and heifers lay down more in the strawyard compared to cubicle housing (P < 0.01) and cows were more aggressive than heifers in both housing systems (P < 0.001 and P < 0.05, respectively). As hypothesized, heifers were more affected by regrouping and cows with more experience settled quicker to their new environment.

Climate change-driven disease in sympatric hosts: Temporal dynamics of parasite burden and proliferative kidney disease in wild brown trout and Atlantic salmon.

Global climate change is altering the abundance and spread of various parasites, which has important consequences not only for host-parasite interactions but also for the relationships between different host species. Here, we focus on the myxozoan endoparasite Tetracapsuloides bryosalmonae that causes temperature-dependent proliferative kidney disease (PKD) in salmonids. We characterized the temporal changes in the parasite load and the severity of PKD signs (renal hyperplasia, haematocrit) in two sympatric populations of wild brown trout (Salmo trutta) and Atlantic salmon (Salmo salar). We found that both the parasite load and disease signs vary considerably between individuals, species, rivers and sampling periods. We showed that Atlantic salmon was able to slow down the initial parasite proliferation rate and subsequently tolerate high parasite burden without obvious disease signs. In contrast, the initial parasite proliferation rate was much higher in brown trout, which was followed by the development of severe PKD signs. Thus, the speed of parasite proliferation, rather than the absolute number of the parasites in the host kidney, may play an important role in interspecific variation in PKD susceptibility. To conclude, this study illustrates the usefulness of temporal perspective for understanding host defence mechanisms and climate change-mediated impacts in the wild.

Antimicrobial Selection for the Treatment of Clinical Mastitis and the Efficacy of Penicillin Treatment Protocols in Large Estonian Dairy Herds.

Clinical mastitis (CM) is the most common microbial disease treated in dairy cows. We analyzed the antimicrobial usage in cows with CM (n = 11,420) in large dairy herds (n = 43) in Estonia. CM treatment data were collected during a 12-month study period. The antimicrobial usage was observed during the 21 days from the initiation of treatment, and the incidence of antimicrobial-treated CM was calculated for each study herd. The effect of intramammary (IMM), systemic, and combined (systemic and IMM) penicillin treatment of CM on the post-treatment somatic cell count (SCC) was analyzed using the treatment records of 2222 cows from 24 herds with a mixed multivariable linear regression model. The median incidence of antimicrobial-treated CM was 35.8 per 100 cow-years. Procaine benzylpenicillin and marbofloxacin were used in 6103 (35.5%, 95% CI 34.8-36.2) and 2839 (16.5%, 95% CI 16.0-17.1) CM treatments, respectively. Post-treatment SCC was higher after IMM penicillin therapy compared to systemic or combination therapy. Treatment of CM usually included first-choice antimicrobials, but different antimicrobial combinations were also widely used. The effect of procaine benzylpenicillin to post-treatment SCC was dependent on the administration route, cow parity, and days in milk. Further studies should evaluate the factors affecting veterinarians' choice of antimicrobial used in the treatment of CM.

Hearing Problems Among the Members of the Defence Forces in Relation to Personal and Occupational Risk Factors.

INTRODUCTION: The Defence Forces' members are exposed to high-level noise that increases their risk of hearing loss (HL). Besides military noise, the other risk factors include age and gender, ototoxic chemicals, vibration, and chronic stress. The current study was designed to study the effects of personal, work conditions-related risk factors, and other health-related traits on the presence of hearing problems. MATERIALS AND METHODS: A cross-sectional study among active military service members was carried out. Altogether, 807 respondents completed a questionnaire about their health and personal and work-related risk factors in indoor and outdoor environments. The statistical analysis was performed using statistical package of social sciences (descriptive statistics) and R (correlation and regression analysis) software. RESULTS: Almost half of the active service members reported HL during their service period. The most important risk factors predicting HL in the military appeared to be age, gender, and service duration. Also, working in a noisy environment with exposure to technological, vehicle, and impulse noise shows a statistically significant effect on hearing health. Moreover, we could identify the effect of stress on tinnitus and HL during the service period. Most importantly, active service members not using hearing protectors, tend to have more tinnitus than those who use it. CONCLUSIONS: The members of the Defence Forces experience noise from various sources, most of it resulting from outdoor activities. Personal and work conditions-related risk factors as well as stress increase the risk of hearing problems.

The Effect of Climate Parameters on Sheep Preferences for Outdoors or Indoors at Low Ambient Temperatures.

Threshold temperatures for cold stress in sheep are not well understood, the available literature is somewhat dated and reports relate to winter temperatures that are relatively benign. Sheep's preferences for outdoor versus indoor environments, when given free access to both, were investigated in the winter period at temperatures as low as -23 °C. Two sheep farms, one with access to a permanent uninsulated barn and one with a polytunnel shelter, both with free access to an outdoor area, were used. Observations were made with a camera positioned to register numbers of sheep outdoors and indoors, with one image taken hourly over twenty-four hours. The sheep clearly preferred to be outdoors; on all occasions the majority of the sheep were outdoors. There was, however, a significant decrease, albeit small, in the numbers of sheep choosing to be outdoors at lower temperatures (p < 0.001), higher relative humidity (p < 0.001) and greater wind chill (p < 0.001). Therefore, even at cooler temperatures than reported previously, sheep are motivated to be outdoors rather than indoors. It is not implicitly good for their welfare, and may not be true for lambs and shorn sheep, but accessing an outdoor area appears to be what they choose to do when given the choice.

Nitrite and nitrate content in meat products and estimated nitrite intake by the Estonian children.

Public interest in nitrate and nitrite content reduction in meat products has increased; therefore, the aim of the present study was to determine nitrate and nitrite levels in commercial meat products as the main source of added nitrites, and to estimate their dietary intake by children. The intake of nitrite from processed meat products and drinking water by Estonian children was estimated. Daily intake estimations were based on the food consumption data of the National Institute for Health Development. In addition, nitrite/nitrate concentrations of meat and processed meat products were measured using a liquid chromatographic method with UV detection. Mean nitrite intakes among 1087 studied children were 0.015 and 0.016 mg kg-1 b.w. day-1, respectively, among children aged 12-35 months and 3-10 years. Acceptable daily intake (ADI) of 0.07 mg nitrite kg-1 b.w. day-1 was exceeded in 3.1% of children, being more prevalent in the younger age group. Considering the consumption of processed meat and drinking water, mean nitrite intakes in the younger and the older age groups were, respectively, 21.9% and 22.9% of the ADI. Study results indicated that over a period of 10 years, children's dietary nitrite intake from processed meat products has declined, which is probably caused by changes in food preferences and decreased usage of nitrite in cured meat products by meat industries.

Dynamics of pertussis toxin IgG after symptomatic pertussis in children and adults.

BACKGROUND AND AIM: The knowledge of dynamics of pertussis toxin (PT)-IgG after pertussis and the appropriate diagnostic cut-off value is limited. We aimed to describe the dynamics of PT-IgG in children and adults up to three years after symptomatic pertussis. METHODS: Patients with persistent cough of unknown aetiology were prospectively enrolled 2012-2014. Pertussis was confirmed by culture, PCR and/or serology. The follow-up samples were taken 4-6 weeks, 1, 2 and 3 years after enrolment. PT-IgG kinetics was described by biexponential model. RESULTS: Pertussis was diagnosed in 22 patients [median (IQR) age 17.7 (8.4-38.6) years]. Adults compared with children had higher peak of the PT-IgG 397 (IQR 374-518) vs 292 (200-363), p = 0.007, longer time to reach peak PT-IgG 16.4 (IQR 15.6-16.8) days vs 13.3 (13.2-13.4) days, p=<0.001 and shorter PT-IgG half-life 24 days (IQR 20-40) and 364 days (IQR 359-486) p < 0.001. CONCLUSION: After symptomatic pertussis, adults and children have different dynamics of PT-IgG. Clinical trial registry: Not applicable.

Uterine Fluid Proteins for Minimally Invasive Assessment of Endometrial Receptivity.

CONTEXT: Clinically used endometrial (EM) receptivity assays are based on transcriptomic patterning of biopsies at midsecretory endometrium (MSE) to identify the possible displacement or disruption of window of implantation (WOI) in patients with recurrent implantation failure (RIF). However, biopsies are invasive and cannot be performed in the same cycle with in vitro fertilization embryo transfer, while uterine fluid (UF) analysis is considered minimally invasive and can immediately precede embryo transfer. OBJECTIVE: To determine whether UF proteome can be used for WOI monitoring and whether it would highlight the etiology of RIF. PATIENTS: Paired early secretory endometrial (ESE) and MSE UF samples from six fertile control women for discovery, and an additional 11 paired ESE/MSE samples from controls and 29 MSE samples from RIF patients for validation. RESULTS: Using discovery mass spectrometry (MS) proteomics we detected 3158 proteins from secretory phase UF of which 367 undergo significant (q < 0.05) proteomic changes while transitioning from ESE to MSE. Forty-five proteins were further validated with targeted MS, and 21 were found to display similar levels between control ESE and RIF MSE, indicating displacement of the WOI. A panel of PGR, NNMT, SLC26A2 and LCN2 demonstrated specificity and sensitivity of 91.7% for distinguishing MSE from ESE samples. The same panel distinguished control MSE samples from RIF MSE with a 91.7% specificity and 96.6% sensitivity. CONCLUSION: UF proteins can be used for estimating uterine receptivity with minimal invasiveness. Women with RIF appear to have altered MSE UF profiles that may contribute to their low IVF success rate.

Pesticide residues in beehive matrices are dependent on collection time and matrix type but independent of proportion of foraged oilseed rape and agricultural land in foraging territory.

Pesticide residues in bee products is still a major issue. However, the relations to botanical source and land use characteristics are not clear. The large variability of residues detected questions the suitability of bee-collected- and other hive materials as indicators for environmental contamination. The aim of our study was to clarify whether different beehive matrices contain similar pesticide residues, and how these are correlated with forage preferences and land use types in foraging areas. We tested bee-collected pollen, beebread, honey, nurse bees and honey bee larvae for the presence of concurrently used agricultural pesticides in Estonia. Samples were collected at the end of May and mid-July to include the main crop in northern region - winter and spring oilseed rape (Brassica napus). We saw that different beehive matrices contained various types of pesticide residues in different proportions: pollen and beebread tended to contain more insecticides and fungicides, whereas herbicides represented the primary contaminant in honey. The variations were related to collection year and time but were not related to crops as basic forage resource nor the land use type. We found few positive correlations between amount of pesticides and proportion of pollen from any particular plant family. None of these correlations were related to any land-use type. We conclude that pesticide residues in different honey bee colony components vary largely in amount and composition. The occurrence rate of pesticide residues was not linked to any particular crop.